The emerging landscape of spatial profiling technologies.
PMID:35859028
A commercial ARHGEF17/TEM4 antibody cross-reacts with Nuclear Mitotic Apparatus protein 1 (NuMA).
PMID:35776709
Self-supervised learning of cell type specificity from immunohistochemical images.
PMID:35758799
The Progression Related Gene RAB42 Affects the Prognosis of Glioblastoma Patients.
PMID:35741652
Manual Immunofluorescence of Formalin-Fixed Paraffin-Embedded Human Tumor Tissues.
PMID:35737240
The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding.
PMID:35563440
MALDI-TOF-MS-Based Identification of Monoclonal Murine Anti-SARS-CoV-2 Antibodies within One Hour.
PMID:35466280
Development of an immunohistochemical assay for Siglec-15.
PMID:35459795
Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence.
PMID:35163349
Loss of MAGEC3 Expression Is Associated with Prognosis in Advanced Ovarian Cancers.
PMID:35158998
Semi-automated validation and quantification of CTLA-4 in 90 different tumor entities using multiple antibodies and artificial intelligence.
PMID:35091676
Reverse phase protein arrays in acute leukemia: investigative and methodological challenges.
PMID:34965151
Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library.
PMID:34946736
Large-Scale Tissue Microarray Evaluation Corroborates High Specificity of High-Level Arginase-1 Immunostaining for Hepatocellular Carcinoma.
PMID:34943588
Rhesus Macaque CODEX Multiplexed Immunohistochemistry Panel for Studying Immune Responses During Ebola Infection.
PMID:34938283
Single-synapse analyses of Alzheimer's disease implicate pathologic tau, DJ1, CD47, and ApoE.
PMID:34910503
Carboxypeptidase A1 (CPA1) Immunohistochemistry Is Highly Sensitive and Specific for Acinar Cell Carcinoma (ACC) of the Pancreas.
PMID:34889867
Spatial mapping of protein composition and tissue organization: a primer for multiplexed antibody-based imaging.
PMID:34811556
Misidentified biomedical resources: Journal guidelines are not a quick fix.
PMID:34807460
PharmacoSTORM nanoscale pharmacology reveals cariprazine binding on Islands of Calleja granule cells.
PMID:34764251
Validation of antibodies: Lessons learned from the Common Fund Protein Capture Reagents Program.
PMID:34757791
Localisation of cryptochrome 2 in the avian retina.
PMID:34677638
Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome.
PMID:34672947
AT-rich interaction domain 5A regulates the transcription of interleukin-6 gene in prostate cancer cells.
PMID:34633095
Subcellular proteomics.
PMID:34549195
Best Practices for Technical Reproducibility Assessment of Multiplex Immunofluorescence.
PMID:34532339
Flow cytometric analysis of equine bronchoalveolar lavage fluid cells in horses with and without severe equine asthma.
PMID:34521286
Fit-for-purpose based testing and validation of antibodies to amino- and carboxy-terminal domains of cannabinoid receptor 1.
PMID:34453219
Non-Specific Binding and Cross-Reaction of ELISA: A Case Study of Porcine Hemoglobin Detection.
PMID:34441486
A ChIP-exo screen of 887 Protein Capture Reagents Program transcription factor antibodies in human cells.
PMID:34426512
BRCA1 Antibodies Matter.
PMID:34421362
Recombinant antibodies and their use for food immunoanalysis.
PMID:34417836
Measuring expression heterogeneity of single-cell cytoskeletal protein complexes.
PMID:34404787
Pattern of placental alkaline phosphatase (PLAP) expression in human tumors: a tissue microarray study on 12,381 tumors.
PMID:34363325
A single-cell type transcriptomics map of human tissues.
PMID:34321199
Asporin represses gastric cancer apoptosis via activating LEF1-mediated gene transcription independent of β-catenin.
PMID:34127813
Mass Spectrometry-Based De Novo Sequencing of Monoclonal Antibodies Using Multiple Proteases and a Dual Fragmentation Scheme.
PMID:34121409
Bead-Based Assays for Validating Proteomic Profiles in Body Fluids.
PMID:34115352
Highly Multiplexed Phenotyping of Immunoregulatory Proteins in the Tumor Microenvironment by CODEX Tissue Imaging.
PMID:34093591
Considerations on Using Antibodies for Studying the Dynorphins/Kappa Opioid Receptor System.
PMID:34085120
Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brains.
PMID:34082785
The Selectivity of Molecularly Imprinted Polymers.
PMID:34071653
Proteomics, Personalized Medicine and Cancer.
PMID:34063807
Antibody Watch: Text mining antibody specificity from the literature.
PMID:34043624
Validation of Isoform- and Mutation-Specific RAS Antibodies.
PMID:33977472
High-specificity antibodies and detection methods for quantifying phosphorylated tau from clinical samples.
PMID:33928234
A Family Affair: Addressing the Challenges of Factor H and the Related Proteins.
PMID:33868311
Epitope-directed monoclonal antibody production using a mixed antigen cocktail facilitates antibody characterization and validation.
PMID:33824395
SUR1, newly expressed in astrocytes, mediates neuropathic pain in a mouse model of peripheral nerve injury.
PMID:33788643
Effects of High Levels of Dietary Linseed Oil on the Growth Performance, Antioxidant Capacity, Hepatic Lipid Metabolism, and Expression of Inflammatory Genes in Large Yellow Croaker (Larimichthys crocea).
PMID:33679441
Spatially Resolved Mass Spectrometry at the Single Cell: Recent Innovations in Proteomics and Metabolomics.
PMID:33656885
Spatiotemporal dissection of the cell cycle with single-cell proteogenomics.
PMID:33627808
High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics.
PMID:33619305
Tissue Distribution of ACE2 Protein in Syrian Golden Hamster (Mesocricetus auratus) and Its Possible Implications in SARS-CoV-2 Related Studies.
PMID:33568991
Power to the protein: enhancing and combining activities using the Spy toolbox.
PMID:33552459
Biomarker Discovery in Patients with Immunotherapy-Treated Melanoma with Imaging Mass Cytometry.
PMID:33504554
Immunoaffinity-Based Liquid Chromatography Mass Spectrometric Assay to Accurately Quantify the Protein Concentration of HMGB1 in EDTA Plasma.
PMID:33420996
SALL4 controls cell fate in response to DNA base composition.
PMID:33406384
TGFBR3L-An Uncharacterised Pituitary Specific Membrane Protein Detected in the Gonadotroph Cells in Non-Neoplastic and Tumour Tissue.
PMID:33396509
Chronic Ethanol Exposure Potentiates Cholinergic Neurotransmission in the Basolateral Amygdala.
PMID:33385490
Induction and repression effects on CYP and transporter protein abundance by azole mixture uptake in rat liver.
PMID:33343269
Aptamer-Based Western Blot for Selective Protein Recognition.
PMID:33195056
Development, Screening, and Validation of Camelid-Derived Nanobodies for Neuroscience Research.
PMID:33185319
Enhanced Validation of Antibodies Enables the Discovery of Missing Proteins.
PMID:33170010
ACE2: Evidence of role as entry receptor for SARS-CoV-2 and implications in comorbidities.
PMID:33164751
Recombinant Antibodies in Basic Neuroscience Research.
PMID:33151027
Toll-Like Receptor 3 in Solid Cancer and Therapy Resistance.
PMID:33147700
The Human Protein Atlas-Spatial localization of the human proteome in health and disease.
PMID:33146890
The Y Chromosome: A Complex Locus for Genetic Analyses of Complex Human Traits.
PMID:33137877
SON and SRRM2 are essential for nuclear speckle formation.
PMID:33095160
A brief guide to good practices in pharmacological experiments: Western blotting.
PMID:33087837
A high-stringency blueprint of the human proteome.
PMID:33067450
Marking vertebrates langerhans cells, from fish to mammals.
PMID:33066843
A synthetic mimic of phosphodiesterase type 5 based on corona phase molecular recognition of single-walled carbon nanotubes.
PMID:33055208
Animal-free alternatives and the antibody iceberg.
PMID:33046876
SAMHD1 phosphorylation and cytoplasmic relocalization after human cytomegalovirus infection limits its antiviral activity.
PMID:32986788
Consistency and variation of protein subcellular location annotations.
PMID:32935893
Research on the Human Proteome Reaches a Major Milestone: >90% of Predicted Human Proteins Now Credibly Detected, According to the HUPO Human Proteome Project.
PMID:32931287
Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting.
PMID:32864552
Genetics meets proteomics: perspectives for large population-based studies.
PMID:32860016
Antibody validation for protein expression on tissue slides: a protocol for immunohistochemistry.
PMID:32852223
ACE2 Protein Landscape in the Head and Neck Region: The Conundrum of SARS-CoV-2 Infection.
PMID:32824830
Generation and validation of recombinant antibodies to study human aminoacyl-tRNA synthetases.
PMID:32817337
ACE2 & TMPRSS2 Expressions in Head & Neck Tissues: A Systematic Review.
PMID:32816230
The Antibody Society's antibody validation webinar series.
PMID:32748696
Mapping the nucleolar proteome reveals a spatiotemporal organization related to intrinsic protein disorder.
PMID:32744794
Profiles of histidine-rich glycoprotein associate with age and risk of all-cause mortality.
PMID:32737166
The protein expression profile of ACE2 in human tissues.
PMID:32715618
High fat diet causes distinct aberrations in the testicular proteome.
PMID:32678325
Gene expression and in situ protein profiling of candidate SARS-CoV-2 receptors in human airway epithelial cells and lung tissue.
PMID:32675206
Facets of individual-specific health signatures determined from longitudinal plasma proteome profiling.
PMID:32629387
Proximity Ligation Assay as a Tool for Antibody Validation in Human Tissues.
PMID:32602410
The effect of journal guidelines on the reporting of antibody validation.
PMID:32547887
Production and characterization of a monoclonal antibody against the sialidase of Gardnerella vaginalis using a synthetic peptide in a MAP8 format.
PMID:32462244
Addressing selective reporting of experiments through predefined exclusion criteria.
PMID:32441650
Overcoming off-targets: assessing Western blot signals for Bcnt/Cfdp1, a tentative component of the chromatin remodeling complex.
PMID:32432658
The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation.
PMID:32414858
Blood-derived biomarkers correlate with clinical progression in Duchenne muscular dystrophy.
PMID:32390640
Expression of the COVID-19 receptor ACE2 in the human conjunctiva.
PMID:32374427
Engineered Ovarian Cancer Cell Lines for Validation of CAR T Cell Function.
PMID:32293122
A recombinant antibody toolbox for Dictyostelium discoideum.
PMID:32276653
Fast Confirmation of Antibody Identity by MALDI-TOF MS Fingerprints.
PMID:32224944
An array of 60,000 antibodies for proteome-scale antibody generation and target discovery.
PMID:32195335
Strategy for Development of Site-Specific Ubiquitin Antibodies.
PMID:32154221
A high sensitivity ZENK monoclonal antibody to map neuronal activity in Aves.
PMID:31969617
Statin use, HMGCR expression, and breast cancer survival - The Malmö Diet and Cancer Study.
PMID:31953433
Antibody validation for Western blot: By the user, for the user.
PMID:31819006
Validation of antibodies for the specific detection of human TRPA1.
PMID:31811235
Anything You Can Do, I Can Do Better: Can Aptamers Replace Antibodies in Clinical Diagnostic Applications?
PMID:31801185
Specificity of a rodent alpha(α)6 nicotinic acetylcholine receptor subunit antibody.
PMID:31786649
CD Maps-Dynamic Profiling of CD1-CD100 Surface Expression on Human Leukocyte and Lymphocyte Subsets.
PMID:31708916
Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.
PMID:31612854
CTLA-4 Immunohistochemistry and Quantitative Image Analysis for Profiling of Human Cancers.
PMID:31609157
Mass Spectrometry-Based Plasma Proteomics: Considerations from Sample Collection to Achieving Translational Data.
PMID:31573204
A call to adopt a "fit for purpose" approach to antibody validation for flow cytometry analyses of stem cell models and beyond.
PMID:31559827
Multiplexed analysis of the secretin-like GPCR-RAMP interactome.
PMID:31555726
Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging.
PMID:31534232
The supply chain of human pancreatic β cell lines.
PMID:31478912
Disrupted hippocampal growth hormone secretagogue receptor 1α interaction with dopamine receptor D1 plays a role in Alzheimer's disease.
PMID:31413143
The ABCD database: a repository for chemically defined antibodies.
PMID:31410491
Anticancer Immunotherapy by MFAP5 Blockade Inhibits Fibrosis and Enhances Chemosensitivity in Ovarian and Pancreatic Cancer.
PMID:31332047
Benchmarking subcellular localization and variant tolerance predictors on membrane proteins.
PMID:31307390
A validated collection of mouse monoclonal antibodies to human glycosyltransferases functioning in mucin-type O-glycosylation.
PMID:31172184
Systematic assessment of antibody selectivity in plasma based on a resource of enrichment profiles.
PMID:31171813
Circulating Levels of Interferon Regulatory Factor-5 Associates With Subgroups of Systemic Lupus Erythematosus Patients.
PMID:31156624
HOT or not: examining the basis of high-occupancy target regions.
PMID:31114922
On the Necessity of Validating Antibodies in the Immunohistochemistry Literature.
PMID:31080409
A Cautionary Tale: Endogenous Biotinylated Proteins and Exogenously-Introduced Protein A Cause Antibody-Independent Artefacts in Western Blot Studies of Brain-Derived Proteins.
PMID:31019379
Multiplex protein detection on circulating tumor cells from liquid biopsies using imaging mass cytometry.
PMID:30906572
Designing a rigorous microscopy experiment: Validating methods and avoiding bias.
PMID:30894402
Multiplexed (18-Plex) Measurement of Signaling Targets and Cytotoxic T Cells in Trastuzumab-Treated Patients using Imaging Mass Cytometry.
PMID:30796036
Understanding of researcher behavior is required to improve data reliability.
PMID:30715291
The highly GABARAP specific rat monoclonal antibody 8H5 visualizes GABARAP in immunofluorescence imaging at endogenous levels.
PMID:30679523
A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain.
PMID:30667360
Spatial proteomics: a powerful discovery tool for cell biology.
PMID:30659282
The 3rd Antibody Validation meeting: Bath UK 20-21 st September 2018.
PMID:30647913
Characterization of single microvesicles in plasma from glioblastoma patients.
PMID:30561734
In focus in HCB.
PMID:30560288
Correlative Light-Electron Microscopy detects lipopolysaccharide and its association with fibrin fibres in Parkinson's Disease, Alzheimer's Disease and Type 2 Diabetes Mellitus.
PMID:30429533
AKT inhibition-mediated dephosphorylation of TFE3 promotes overactive autophagy independent of MTORC1 in cadmium-exposed bone mesenchymal stem cells.
PMID:30324847
Enhanced validation of antibodies for research applications.
PMID:30297845
Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies.
PMID:30244833
Proteomic Approaches for the Discovery of Biofluid Biomarkers of Neurodegenerative Dementias.
PMID:30200280
High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB).
PMID:30199009
Sulfonylurea Receptor 1, Transient Receptor Potential Cation Channel Subfamily M Member 4, and KIR6.2:Role in Hemorrhagic Progression of Contusion.
PMID:30160201
Progress on Identifying and Characterizing the Human Proteome: 2018 Metrics from the HUPO Human Proteome Project.
PMID:30099871
Antibody validation: a view from the mountains.
PMID:30086383
A Computational Synaptic Antibody Characterization Tool for Array Tomography.
PMID:30065633
Validating Antibodies for Quantitative Western Blot Measurements with Microwestern Array.
PMID:30054510
Directed evolution of a picomolar-affinity, high-specificity antibody targeting phosphorylated tau.
PMID:29899114
Ca2+-binding protein NECAB2 facilitates inflammatory pain hypersensitivity.
PMID:29893745
Mouse-to-mouse variation in maturation heterogeneity of smooth muscle cells.
PMID:29796562
Kir6.2, the Pore-Forming Subunit of ATP-Sensitive K+ Channels, Is Overexpressed in Human Posttraumatic Brain Contusions.
PMID:29737232
A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors.
PMID:29638227
Current applications of antibody microarrays.
PMID:29507545
Ten Basic Rules of Antibody Validation.
PMID:29467569
Targeting RAS-driven human cancer cells with antibodies to upregulated and essential cell-surface proteins.
PMID:29359686
Guidelines for authors and reviewers on antibody use in physiology studies.
PMID:29351459
Goals and practicalities of immunoblotting and immunohistochemistry: A guide for submission to the British Journal of Pharmacology.
PMID:29350411
RNAscope in situ hybridization confirms mRNA integrity in formalin-fixed, paraffin-embedded cancer tissue samples.
PMID:29212158
MS Western, a Method of Multiplexed Absolute Protein Quantification is a Practical Alternative to Western Blotting.
PMID:29192002
A multiregional proteomic survey of the postnatal human brain.
PMID:29184206
A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells.
PMID:29184000
A recombinant O-polysaccharide-protein conjugate approach to develop highly specific monoclonal antibodies to Shiga toxin-producing Escherichia coli O157 and O145 serogroups.
PMID:28981517
Pituitary adenylate cyclase-activating polypeptide receptors in the trigeminovascular system: implications for migraine.
PMID:28977676
Protein purification and analysis: next generation Western blotting techniques.
PMID:28974114
Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies.
PMID:28951536
The human protein atlas: A spatial map of the human proteome.
PMID:28940711
Quantitative dot blot analysis (QDB), a versatile high throughput immunoblot method.
PMID:28938578
The Human Plasma Proteome Draft of 2017: Building on the Human Plasma PeptideAtlas from Mass Spectrometry and Complementary Assays.
PMID:28938075
Progress on the HUPO Draft Human Proteome: 2017 Metrics of the Human Proteome Project.
PMID:28853897
Development of an Affimer-antibody combined immunological diagnosis kit for glypican-3.
PMID:28852111
Intracellular Proteolysis of Progranulin Generates Stable, Lysosomal Granulins that Are Haploinsufficient in Patients with Frontotemporal Dementia Caused by GRN Mutations.
PMID:28828399
Reactivity of human AGO2 monoclonal antibody 11A9 with the SWI/SNF complex: A case study for rigorously defining antibody selectivity.
PMID:28779093
Evaluating Commercially Available Antibodies for Rat α7 Nicotinic Acetylcholine Receptors.
PMID:28763248
Novel sensitive monoclonal antibody based competitive enzyme-linked immunosorbent assay for the detection of raw and processed bovine beta-casein.
PMID:28759641
A cardinal sin when researching neuropsin/KLK8: Thou shalt validate antibodies.
PMID:28732191
Choose and Use Your Chemical Probe Wisely to Explore Cancer Biology.
PMID:28697345
Insufficient antibody validation challenges oestrogen receptor beta research.
PMID:28643774
Reproducibility2020: Progress and priorities.
PMID:28620458
Neuronal circadian clock protein oscillations are similar in behaviourally rhythmic forager honeybees and in arrhythmic nurses.
PMID:28615472
Last rolls of the yoyo: Assessing the human canonical protein count.
PMID:28529709
Affinity Proteomics Exploration of Melanoma Identifies Proteins in Serum with Associations to T-Stage and Recurrence.
PMID:28433799
Protein Array-based Approaches for Biomarker Discovery in Cancer.
PMID:28392481
DOSCATs: Double standards for protein quantification.
PMID:28368040
The challenges with the validation of research antibodies.
PMID:28357047
Nuclear IRF-1 expression as a mechanism to assess "Capability" to express PD-L1 and response to PD-1 therapy in metastatic melanoma.
PMID:28331615
Epitopes of anti-RIFIN antibodies and characterization of rif-expressing Plasmodium falciparum parasites by RNA sequencing.
PMID:28233866
Current Challenges and Limitations in Antibody-Based Detection of Citrullinated Histones.
PMID:27933065