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qPrimerDepot

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qPrimerDepot is a primer database for RT-PCR analysis of greater than 99% of human and mouse RefSeq genes. These primers sets are designed to be used under uniform annealing temperatures to facilitate their application in large scale high throughput assays. For intron-bearing genes, over 90% of the primer sets are designed to produce amplicons bridging exon:exon junctions of intron-bearing genes. Thus, preserving the signal to noise ratio against genomic contamination. The database is available on line at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/.

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qPrimerDepot is a primer database for RT-PCR analysis of greater than 99% of human and mouse RefSeq genes. These primers sets are designed to be used under uniform annealing temperatures to facilitate their application in large scale high throughput assays. For intron-bearing genes, over 90% of the primer sets are designed to produce amplicons bridging exon:exon junctions of intron-bearing genes. Thus, preserving the signal to noise ratio against genomic contamination. The database is available on line at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/.

metasource: Nucleic Acid Research database catalogue
version: extracted_at: 2022-11-04T11:16:25.468657

Webpage:

http://primerdepot.nci.nih.gov/

Webpage

More detailed information about this field from each metasource.

http://primerdepot.nci.nih.gov/

metasource: Nucleic Acid Research database catalogue
version: extracted_at: 2022-11-04T11:16:25.468657

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other molecular biology databases
metasource: Nucleic Acid Research database catalogue
version: extracted_at: 2022-11-04T11:16:25.468657

molecular probes and primers
metasource: Nucleic Acid Research database catalogue
version: extracted_at: 2022-11-04T11:16:25.468657

molecular biology molecular probes and primers

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Data is amalgamated and only retrieved periodically.
Some of the resources used by the system:

® FAIRsharing.org
® Bioontology/Bioportal Terms
® Nucleic Acid Research - Database
® re3data.org
® bio.tools
® PubMed - Courtesy of the U.S. National Library of Medicine